JCM Catalogue

Thiobacillus ferrooxidans Temple and Colmer 1951
Taxonomy in NCBI database: Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales; Thiobacillaceae.
3863 <-- Y. Katayama THI132 <-- ATCC 19859 <-- NCIB 9490 <-- P. Trussell.
Accessioned in 1985.
=ATCC 19859 =BCRC 13033 =NCIMB 9490.
Thiobacillus ferrooxidans.
Medium: 92;  Temperature: 30°C; Rehydration fluid: 92.   more information +  Full / Short version of the cataloguepage top
Source: Mine water.
Fatty acid: C16:0, C16:1+ΔC17:0, C18:1+ΔC19:0, 3OH-C14:0 [2142].
Quinone: Q-8 [2142].
G+C (mol%): 58 (Tm) [2287].
DNA-DNA relatedness: [2287].
Other taxonomic data: Polyamine [4995].
Taxonomy: Group III-3 [2142].
BacDive ID: 164860.
Publication(s) using this strain [A04038, A05058, A07220, A12397, A18182, A20090].
Patent publication(s) using this strain [2019-154435, 2022-146325].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Not applicable -
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information How to revive and cultivate Acidithiobacillus ferrooxidans.   
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
3865 <-- Y. Katayama THI135 <-- H. Kakuta.
Accessioned in 1985.
Thiobacillus ferrooxidans.
Medium: 92;  Temperature: 30°C; Rehydration fluid: 92.   more information +
Source: Acid mine water of a mine at Shimokawa, Hokkaido.
Fatty acid: C16:0, C16:1+ΔC17:0, C18:1+ΔC19:0, 3OH-C14:0 [2142].
Quinone: Q-8 [2142].
G+C (mol%): 57.4 (Tm) [2142].
DNA-DNA relatedness: [2143].
Phylogeny: 16S rRNA gene (FN686785), recA (FN687438), atpD (FN689434), nifH (FN688748), rusB (FN690755), iro (FN688774) [8515].
Other taxonomic data: 16S-23S rRNA ITS (FN687157) [8515].
Taxonomy: Group III-3 [2142].
BacDive ID: 164861.
Publication(s) using this strain [A11287].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Not applicable -
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information How to revive and cultivate Acidithiobacillus ferrooxidans.   
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
7812 <-- N. Wakao AIU 002.
Accessioned in 1990.
Thiobacillus ferrooxidans.
Medium: 92;  Temperature: 30°C; Rehydration fluid: 92.   more information +
Source: Acid mine water from Matsuo sulfur and iron sulfide mine on Hachimantai volcano, Iwate Pref., Japan [4784].
Biochemistry/Physiology: [4784].
Fatty acid: [4784].
Quinone: Q-8 [4784].
G+C (mol%): 56 (Tm) [4784].
Phylogeny: 16S rRNA gene (FN686782), recA (FN687437), atpD (FN689432), nifH (FN688747), rusB (FN688763), iro (FN688774) [8515].
Other taxonomic data: 16S-23S rRNA ITS (FN687156) [8515].
BacDive ID: 165617.
Publication(s) using this strain [A11287, A13155, B13341, B16238, A16527, A16531].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Not applicable -
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information How to revive and cultivate Acidithiobacillus ferrooxidans.   
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control but note that the authenticity has not yet been checked by gene sequencing. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
17309 <-- T. Itoh <-- K. B. Hallbarg JCM 7811-P1 <-- JCM 7811 <-- N. Wakao AIU 001.
Accessioned in 2010.
Thiobacillus ferrooxidans.
Medium: 92;  Temperature: 30°C; Rehydration fluid: 92.   more information +
Source: Clonal isolate obtained from the JCM 7811 culture originating in the Matsuo sulfur and iron sulfide mine, Japan [8515].
Biochemistry/Physiology: [8515].
Phylogeny: 16S rRNA gene (FN686780), recA (FN687435), atpD (FN689430), nifH (FN688745), rusA (FN688761), hip (FN688772) [8515].
Other taxonomic data: 16S-23S rRNA ITS (FN687154) [8515].
More information: Isolated from JCM 7811 as JCM 7811-P1 [8515].
BacDive ID: 162005.
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Not applicable -
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information How to revive and cultivate Acidithiobacillus ferrooxidans.   
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
18981 <-- D. B. Johnson <-- V. Bonnefoy <-- D. E. Rawlings <-- ATCC 33020 <-- H. M. Tsuchiya <-- N. Tomizuka 11Fe.
Accessioned in 2013.
=ATCC 33020 =DSM 29468.
Acidithiobacillus ferrooxidans.
Thiobacillus ferrooxidans.
Type strain [9711].
Medium: 92;  Temperature: 30°C.   more information +
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Source: Ore from Ningyo-toge mine, Okayama, Japan [9350].
Biochemistry/Physiology: [9350,9711].
Fatty acid: [9711].
Quinone: Q-8 [9711].
Polar lipid: [9711].
G+C (mol%): 58 (Tm).
Phylogeny: 16S rRNA gene (AJ278719).
Genome sequence: AP018795.
More information: Leaching of uranium [9350].
NCBI Assembly ID: GCA_003966655 (GenBank), GCF_003966655 (RefSeq).
BacDive ID: 130138.
NCBI Taxonomy ID: 1232575.
Publication(s) using this strain [B13341, A14094, A16527, A18491].
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic F (Frozen culture with dry ice) or C (Actively growing culture on request)
Overseas B (Actively growing culture of a microbial strain that is not preserved as a dried culture / a microbial strain of which dried ampoules are not available)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
17358 <-- D. B. Johnson <-- DSM 2392 <-- A. P. Harrison, Jr. strain m-1 <-- B. Jarvis.
Accessioned in 2010.
=DSM 2392.
Acidithiobacillus ferrooxidans.
Thiobacillus ferrooxidans.
Type strain [8591,8833].
Medium: 861;  Temperature: 37°C.   more information +
Source: Forty-year-old coal refuse, Bevier coal seam, Calloway county, MO, USA [2287].
Biochemistry/Physiology: [2287,8591].
G+C (mol%): 65 (Tm) [2287], 63.1 (Tm) [8591].
DNA-DNA relatedness: [2287].
Phylogeny: 16S rRNA gene (AF387301) [8591].
Genome sequence: PSYR00000000.
NCBI Assembly ID: GCA_003333315 (GenBank), GCF_003333315 (RefSeq).
BacDive ID: 4303.
NCBI Taxonomy ID: 163359.
Publication(s) using this strain [C11059].
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic F (Frozen culture with dry ice) or C (Actively growing culture on request)
Overseas B (Actively growing culture of a microbial strain that is not preserved as a dried culture / a microbial strain of which dried ampoules are not available)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
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