Medium data

Search for medium no. = [861].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

861   ACIDIFERROBACTER MEDIUM

Modified UBS solution (see Medium No. 860)  50.0     ml
MgSO4·7H2O  26.64    g
Sulfur (powder)  1.0     g
Distilled water  885.0     ml
Mix thoroughly except sulfur, adjust pH 1.9 with H2SO4 and autoclave. Steam sulfur for 3 hr on each of 3 successive days. After cooling, add sulfur and the following solutions (filter-sterilized) to the medium:
1.0 M FeSO4 solution (pH 2.0)  10.0     ml
100 mM Potassium tetrathionate solution  5.0     ml
Trace element solution (see Medium No. 748)  10.0     ml