Medium data
Search for medium no. = [861].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
861 ACIDIFERROBACTER MEDIUM
| Modified UBS solution (see Medium No. 860) | 50.0 | ml |
| MgSO4·7H2O | 26.64 | g |
| Sulfur (powder) | 1.0 | g |
| Distilled water | 885.0 | ml |
Mix thoroughly except sulfur, adjust pH 1.9 with H2SO4 and autoclave. Steam sulfur for 3 hr on each of 3 successive days. After cooling, add sulfur and the following solutions (filter-sterilized) to the medium:
| 1.0 M FeSO4 solution (pH 2.0) | 10.0 | ml |
| 100 mM Potassium tetrathionate solution | 5.0 | ml |
| Trace element solution (see Medium No. 748) | 10.0 | ml |
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