Medium data
Search for medium no. = [860].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
860 FERROVUM MEDIUM
| Modified UBS solution (see below) | 50.0 | ml |
| MgSO4·7H2O | 12.32 | g |
| Distilled water | 920.0 | ml |
Mix thoroughly, adjust pH to 2.3 with H2SO4 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
| 1.0 M FeSO4 solution (pH 2.0) | 20.0 | ml |
| Trace element solution (see Medium No. 748) | 10.0 | ml |
Modified UBS solution:
| Na2SO4 | 1.5 | g |
| (NH4)2SO4 | 4.5 | g |
| KCl | 0.5 | g |
| K2HPO4 | 0.5 | g |
| Ca(NO3)2·4H2O | 0.14 | g |
| Distilled water | 1.0 | L |
Comment: For cultivation of JCM 30830, adjust pH of the medium to 1.7.
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