Medium data

Search for medium no. = [860].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

860   FERROVUM MEDIUM

Modified UBS solution (see below)  50.0     ml
MgSO4·7H2O  12.32    g
Distilled water  920.0     ml
Mix thoroughly, adjust pH to 2.3 with H2SO4 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
1.0 M FeSO4 solution (pH 2.0)  20.0     ml
Trace element solution (see Medium No. 748)  10.0     ml
Modified UBS solution:
Na2SO4  1.5     g
(NH4)2SO4  4.5     g
KCl  0.5     g
K2HPO4  0.5     g
Ca(NO3)2·4H2O  0.14    g
Distilled water  1.0     L
Comment: For cultivation of JCM 30830, adjust pH of the medium to 1.7.