Medium data

Search for medium no. = [748].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

748   METALLOSPHAERA CUPRINA MEDIUM

(NH4)2SO4  3.0     g
KH2PO4  0.5     g
MgSO4·7H2O  0.5     g
KCl  0.1     g
Ca(NO3)2  0.01    g
Yeast extract  2.0     g
Tryptone (BD-Difco)  0.5     g
Distilled water  1.0     L
Adjust pH to 3.5 and autoclave. After cooling, aseptically add 2.0 ml of Trace element solution (see below).
Trace element solution (filter-sterilized):
FeCl3·6H2O  1.1     g
CuSO4·5H2O  0.05    g
H3BO3  0.2     g
MnSO4·xH2O  0.2     g
Na2MoO4·2H2O  0.08    g
CoCl2·6H2O  0.06    g
ZnSO4·7H2O  0.09    g
Distilled water  1.0     L