Medium data

Search for medium no. = [1334].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1334 NATRONOARCHAEA MEDIUM II

Neutral base salt medium (see below)	`750.0`	ml
Base soda medium (see Medium No. 1207)	`250.0`	ml

Mix two media autoclaved separately. Aseptically add the following solutions (autoclaved or *filter-sterilized):

1 M MgCl₂ solution	`1.0`	ml
Trace vitamins* (see Medium No. 197)	`5.0`	ml
Trace element solution (see Medium No. 1079)	`1.0`	ml
Se/W solution (see Medium No. 852)	`0.5`	ml
10% Yeast extract solution	`0.2`	ml
10% Soluble starch solution	`10.0`	ml

Neutral base salt medium:

Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with 0.5 M K₂HPO₄ and autoclave.