Medium data

Search for medium no. = [197].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

197   

KH2PO4  0.75    g
K2HPO4  0.75    g
MgCl2·6H2O  0.36    g
NH4Cl  1.0     g
Yeast extract (BD-Difco)  0.1     g
Polypepton (Nihon Pharm. Co.)  0.1     g
Sodium acetate  2.5     g
Trimethylamine·HCl  8.0     g
Trace mineral solution (see below)  9.0     ml
Trace vitamins (see below)  10.0     ml
NaHCO3  0.8     g
NaS2·9H2O  0.5     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  981.0     ml
Mix ingredients except NaHCO3, Trace vitamins, Na2S·9H2O and cysteine·HCl·H2O, and autoclave under a N2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as an 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S·9H2O and cysteine·HCl·H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S·9H2O and cysteine·HCl·H2O solutions, and adjust pH to 6.8.
Trace vitamins:
Biotin  2.0     mg
Folic acid  2.0     mg
Pyridoxine·HCl  10.0     mg
Thiamine·HCl  5.0     mg
Riboflavin  5.0     mg
Nicotinic acid  5.0     mg
Calcium pantothenate  5.0     mg
Vitamin B12  0.1     mg
p-Aminobenzoic acid  5.0     mg
Lipoic acid  5.0     mg
Distilled water  1.0     L
Trace mineral solution:
Nitrilotriacetic acid  4.5     g
FeCl2·4H2O  0.4     g
MnCl2·4H2O  0.1     g
CoCl2·6H2O  0.17    g
ZnCl2  0.1     g
CaCl2·2H2O  0.02    g
H3BO3  0.019   g
Na2MoO4·2H2O  0.01    g
NiCl2·6H2O  0.03    g
Distilled water  1.0     L
Adjust pH to 7.0 with 1 N KOH.