Medium data
Search for medium no. = [1207].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1207 NATRONOARCHAEA MEDIUM WITH GLUCOSAMINE
| Base salt medium (see below) | 875.0 | ml |
| Base soda medium (see below) | 125.0 | ml |
Mix two media autoclaved separately. Aseptically add the following solutions (autoclaved or *filter-sterilized):
| 1 M MgSO4 solution | 1.0 | ml |
| Trace vitamins* (see Medium No. 197) | 5.0 | ml |
| Trace element solution (see Medium No. 1079) | 1.0 | ml |
| 10% Yeast extract solution | 0.2 | ml |
| 10% N-Acetyl-D-glucosamine solution* | 10.0 | ml |
Base salt medium:
| NaCl | 240.0 | g |
| KCl | 5.0 | g |
| K2HPO4 | 2.5 | g |
| NH4Cl | 0.5 | g |
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with 0.5 M K2HPO4 and autoclave.
Base soda medium:
| Na2CO3 | 190.0 | g |
| NaHCO3 | 30.0 | g |
| NaCl | 16.0 | g |
| K2HPO4 | 1.0 | g |
Add components to distilled water and bring volume to 1.0 L. Autoclave.
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