Medium data

Search for medium no. = [1207].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1207   NATRONOARCHAEA MEDIUM WITH GLUCOSAMINE

Base salt medium (see below)  875.0     ml
Base soda medium (see below)  125.0     ml
Mix two media autoclaved separately. Aseptically add the following solutions (autoclaved or *filter-sterilized):
1 M MgSO4 solution  1.0     ml
Trace vitamins* (see Medium No. 197)  5.0     ml
Trace element solution (see Medium No. 1079)  1.0     ml
10% Yeast extract solution  0.2     ml
10% N-Acetyl-D-glucosamine solution*  10.0     ml
Base salt medium:
NaCl  240.0     g
KCl  5.0     g
K2HPO4  2.5     g
NH4Cl  0.5     g
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with 0.5 M K2HPO4 and autoclave.
Base soda medium:
Na2CO3  190.0     g
NaHCO3  30.0     g
NaCl  16.0     g
K2HPO4  1.0     g
Add components to distilled water and bring volume to 1.0 L. Autoclave.