Medium data

Search for medium no. = [1079].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1079 HALANAEROARCHAEUM MEDIUM

Basal mineral NaCl medium:

NaCl	`240.0`	g
K₂HPO₄	`2.5`	g
NH₄Cl	`0.5`	g
HEPES	`7.0`	g

Add components to distilled water and bring volume to 1.0 L. Autoclave and, after cooling, add per liter the following solutions (autoclaved or *filter-sterilized separately):

1 M MgCl₂ solution	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
Trace element solution (see below)	`1.0`	ml
1 M NaHCO₃ solution*	`10.0`	ml

To complete the medium, aseptically and anaerobically distribute the medium into culture vessels containing steam-sterilized sulfur (see below, approximately 1.0 g/L) under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized):

2 M Sodium pyruvate solution*	`10.0`	ml
10% Yeast extract solution	`0.2`	ml
5% Na₂S·9H₂O solution	`5.0`	ml

Sterilization of sulfur:
Steam sulfur for 3 hr, or autoclave at 105C for 1 hr, on each of 3 successive days.
Trace element solution:

EDTA	`5.0`	g
FeSO₄·7H₂O	`2.0`	g
ZnSO₄·7H₂O	`0.10`	g
MnCl₂·4H₂O	`0.03`	g
H₃BO₃	`0.3`	g
CoCl₂·6H₂O	`0.2`	g
CuCl₂·2H₂O	`0.01`	g
NiCl₂·6H₂O	`0.02`	g
Na₂MoO₄·2H₂O	`0.03`	g
Distilled water	`1.0`	L

Adjust pH to 3.6.