Medium data

Search for medium no. = [852].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

852 SPIROCHAETA MEDIUM

KH₂PO₄	`0.1`	g
MgCl₂·6H₂O	`4.0`	g
CaCl₂·2H₂O	`1.0`	g
NH₄Cl	`0.5`	g
NaCl	`25.0`	g
Yeast extract	`1.0`	g
Sucrose	`1.7`	g
Maltose	`1.7`	g
Vitamin solution (see below)	`2.0`	ml
Trace mineral solution (see below)	`1.0`	ml
Se/W solution (see below)	`1.0`	ml
NaHCO₃	`2.0`	g
Resazurin	`1.0`	mg
L-Cysteine·HCl·H₂O	`0.3`	g
Na₂S·9H₂O	`0.3`	g
Distilled water	`1.0`	L

Trace mineral solution:

FeCl₂·4H₂O	`1.99`	g
MnCl₂·4H₂O	`0.198`	g
CoCl₂·6H₂O	`0.13`	g
ZnCl₂	`0.136`	g
CuCl₂·2H₂O	`1.7`	mg
H₃BO₃	`6.2`	mg
Na₂MoO₄·2H₂O	`0.024`	g
NiCl₂·6H₂O	`0.013`	g
AlCl₃	`0.013`	g
Distilled water	`1.0`	L

Se/W solution:

Na₂SeO₃·5H₂O	`1.7`	mg
Na₂WO₄·2H₂O	`3.3`	mg
Distilled water	`1.0`	L

Vitamin solution:

Biotin	`4.9`	mg
Folic acid	`8.8`	mg
Pyridoxine·HCl	`4.1`	mg
Thiamine·HCl	`6.7`	mg
Riboflavin	`7.5`	mg
Nicotinamide	`2.5`	mg
DL-Calcium pantothenate	`9.5`	mg
Vitamin B₁₂	`27.1`	mg
p-Aminobenzoic acid	`2.7`	mg
Lipoic acid	`4.1`	mg
Distilled water	`1.0`	L

For vial cultures, dissolve all compounds except Na₂S·9H₂O and L-cysteine·HCl·H₂O, then, adjust pH to 7.5. Dispense 19 ml of medium into 50-ml vials, purge with N₂-CO₂ (80:20) gas and fix with a stopper. After autoclaving, add filter-sterilized Na₂S·9H₂O and L-cysteine·HCl·H₂O solution to each vial as reducing agents.