JCM Catalogue

Mycobacterium smegmatis (Trevisan 1889) Lehmann and Neumann 1899
Taxonomy in NCBI database: Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae.
5866 <-- NCTC 8159 <-- R. E. Gordon <-- Hagan <-- Alvarez and Tavel strain Cornell 3.
Accessioned in 1986.
=ATCC 19420 =CCUG 21002 =CCUG 21815 =CECT 3020 =CIP 104444 =DSM 43756 =JCM 6386 =KCTC 9108 =NCTC 8159 =NRRL B-14616 =VKM Ac-1239.
Mycobacterium smegmatis.
Type strain [596,12055,12062].
Medium: 26, 386;  Temperature: 37°C; Rehydration fluid: 656.   more information +  Full / Short version of the cataloguepage top
Biochemistry/Physiology: [5666].
G+C (mol%): 67.3 (genome sequence) [12227].
DNA-DNA relatedness: [5666].
Phylogeny: 16S rRNA gene (AJ131761, AJ536041, AY457078) [7312,7733], 5S rRNA [3856], rpoB (AY262735) [8692].
DNA typing: RFLP pattern [5666].
Other taxonomic data: 16S-23S rRNA ITS (AJ291599) [5186]; Mycolic acid [5666].
Taxonomy: [12055].
Genome sequence: LN831039.
NCBI Assembly ID: GCA_001457595 (GenBank), GCF_001457595 (RefSeq).
BacDive ID: 8324.
NCBI Taxonomy ID: 1772.
Genomic DNA is available from RIKEN BRC-DNA Bank: JGD07449.
Publication(s) using this strain [A10218].
 Related information on delivery / use of the strain
Biosafety level 2
Terms and conditions Not applicable -
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
6386 <-- M. Tsukamura 17041 <-- R. J. Wallace <-- ATCC 19420 <-- NCTC 8159 <-- R. E. Gordon <-- Hagan <-- Alvarez and Tavel strain Cornell 3.
Accessioned in 1987.
=ATCC 19420 =CCUG 21002 =CCUG 21815 =CECT 3020 =CIP 104444 =DSM 43756 =JCM 5866 =KCTC 9108 =NCTC 8159 =NRRL B-14616 =VKM Ac-1239.
Mycobacterium smegmatis.
Type strain [596,12055,12062].
Medium: 26, 386;  Temperature: 37°C; Rehydration fluid: 656.   more information +
Biochemistry/Physiology: [5666].
G+C (mol%): 67.3 (genome sequence) [12227].
DNA-DNA relatedness: [5666].
Phylogeny: 16S rRNA gene (AJ131761, AJ536041, AY457078) [7312,7733], 5S rRNA [3856], rpoB (AY262735) [8692].
DNA typing: RFLP pattern [5666].
Other taxonomic data: 16S-23S rRNA ITS (AJ291599) [5186]; Mycolic acid [5666].
Taxonomy: [12055].
Genome sequence: LN831039.
NCBI Assembly ID: GCA_001457595 (GenBank), GCF_001457595 (RefSeq).
NCBI Taxonomy ID: 1772.
Publication(s) using this strain [A08296, A10084, A10218, A18412, A21181, A21526].
Patent publication(s) using this strain [2019-149972].
 Related information on delivery / use of the strain
Biosafety level 2
Terms and conditions Not applicable -
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
20379 <-- IAM 12065 <-- A. Shimazu <-- ATCC 607 <-- R. D. Herrold <-- F. J. Novy (original Koch's strain of "Bacillus tuberculosis").
Accessioned in 2007.
=ATCC 607 =IAM 12065 =IFO 3207 =NBRC 3207 =NRRL B-692.
Mycobacterium smegmatis.
Medium: 26, 386;  Temperature: 37°C; Rehydration fluid: 656.   more information +
Source: Human.
Assay: Antibiotics (Japanese Pharmacopoeia, 14 ed.).
BacDive ID: 8319.
Publication(s) using this strain [A10218, A17162, A17471].
Patent publication(s) using this strain [JP2014-188032A].
 Related information on delivery / use of the strain
Biosafety level 2
Terms and conditions Not applicable -
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
31864 <-- M. Ogawa; Univ. of Occup. & Environ. Health, Japan; J15cs.
Accessioned in 2017.
Mycobacterium smegmatis.
Medium: 26, 386;  Temperature: 37°C; Rehydration fluid: 656.   more information +
Source: Derived from Mycobacterium smegmatis strain Jucho [11438].
Polar lipid: DPG, PE, PIMs [11439].
Other taxonomic data: Mycolic acid [11439], apolar glycopeptidolipids [11439,11441].
More information: Capability of serving as an appropriate host-vector system with pYT923hyg for molecular study of the intracellular growth of M. tuberculosis in phagocytic cells [11438].
BacDive ID: 164140.
 Related information on delivery / use of the strain
Biosafety level 2
Terms and conditions Not applicable -
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
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