Medium data
Search for medium no. = [656].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
656 TRYPTONE-YEAST EXTRACT BROTH (ISP-1) WITH 0.2% MgSO4·7H2O
Tryptone (BD-Difco) | 5.0 | g |
Yeast extract (BD-Difco) | 3.0 | g |
MgSO4·7H2O | 2.0 | g |
Distilled water | 1.0 | L |
Adjust pH to 7.0 - 7.2.
Comment: The premixed powder of Tryptone-yeast extract broth is available from Becton Dickinson & Co. as ISP Medium 1. Add MgSO4·7H2O to the medium before autoclaving.
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