JCM Catalogue

Bacillus simplex Priest et al. 1989
Taxonomy in NCBI database: Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae.
12307 <-- DSM 1321 <-- R. E. Gordon NRS 960 <-- J. R. Porter <-- O. F. Edwards <-- H. J. Cohn.
Accessioned in 2003.
=ATCC 49097 =BCRC 15909 =CCM 7148 =CCUG 28889 =CGMCC 1.3471 =CIP 106934 =DSM 1321 =IFO 15720 =KCTC 3450 =LMG 11160 =NBRC 15720 =NRRL NRS-960 =VTT E-97781.
Bacillus simplex.
Type strain [4089,5532,12816].
Medium: 22;  Temperature: 30°C; Rehydration fluid: 663.   more information +  Full / Short version of the cataloguepage top
Source: Soil [5532].
Biochemistry/Physiology: [5532].
Numerical taxonomy: [5532].
G+C (mol%): 40-41 (Tm) [5532].
Phylogeny: 16S rRNA gene (AB363738, AJ439078, AJ628743, D78478, X60638) [3047].
Taxonomy: [12816].
Genome sequence: BCVO00000000.
NCBI Assembly ID: GCA_001591785 (GenBank), GCF_001591785 (RefSeq).
BacDive ID: 790.
NCBI Taxonomy ID: 1478.
Publication(s) using this strain [A20173, A21119].
Patent publication(s) using this strain [JP2009-284861A, 2021-40614].
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information -
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
28805 <-- K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; T7405-4-2b.
Accessioned in 2016.
Bacillus simplex.
Medium: 475;  Temperature: 28°C; Rehydration fluid: 656.   more information +
Source: Blackish substances on the top surface of north wall (during relocation of the stone chamber) of the Takamatsuzuka Tumulus, Asuka Village, Nara Pref., Japan [11960].
Phylogeny: 16S rRNA gene (LC150661), gyrB (LC150726) [11960].
Taxonomy: Identification [11960].
BacDive ID: 163610.
Publication(s) using this strain [C17567].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Imposed
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
28806 <-- K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; T7409-2-4b.
Accessioned in 2016.
Bacillus simplex.
Medium: 475;  Temperature: 28°C; Rehydration fluid: 656.   more information +
Source: Soil and plaster behind the north wall (during relocation of the stone chamber) of the Takamatsuzuka Tumulus, Asuka Village, Nara Pref., Japan [11960].
Phylogeny: 16S rRNA gene (LC150662), gyrB (LC150727) [11960].
Taxonomy: Identification [11960].
BacDive ID: 163611.
Publication(s) using this strain [C17567].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Imposed
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
28807 <-- K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; T7410-1-5b.
Accessioned in 2016.
Bacillus simplex.
Medium: 475;  Temperature: 28°C; Rehydration fluid: 656.   more information +
Source: Plasters between north wall and ceiling stone (during relocation of the stone chamber) of the Takamatsuzuka Tumulus, Asuka Village, Nara Pref., Japan [11960].
Phylogeny: 16S rRNA gene (LC150663), gyrB (LC150728) [11960].
Taxonomy: Identification [11960].
BacDive ID: 163612.
Publication(s) using this strain [C17567].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Imposed
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
28813 <-- K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; T7413-6-1b.
Accessioned in 2016.
Bacillus simplex.
Medium: 475;  Temperature: 28°C; Rehydration fluid: 656.   more information +
Source: A piece of plaster on the joint space between east wall 3 and north wall (during relocation of the stone chamber) of the Takamatsuzuka Tumulus, Asuka Village, Nara Pref., Japan [11960].
Phylogeny: 16S rRNA gene (LC150667), gyrB (LC150729) [11960].
Taxonomy: Identification [11960].
BacDive ID: 163619.
Publication(s) using this strain [C17567].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Imposed
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
28848 <-- K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; T81119-2-3b.
Accessioned in 2016.
Bacillus simplex.
Medium: 475;  Temperature: 28°C; Rehydration fluid: 656.   more information +
Source: Soil around western area of conservation facility of the Takamatsuzuka Tumulus, Asuka Village, Nara Pref., Japan [11960].
Phylogeny: 16S rRNA gene (LC150681), gyrB (LC150730) [11960].
Taxonomy: Identification [11960].
BacDive ID: 163653.
Publication(s) using this strain [C17567].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Imposed
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
28856 <-- K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; K6203-10-3b.
Accessioned in 2016.
Bacillus simplex.
Medium: 475;  Temperature: 28°C; Rehydration fluid: 656.   more information +
Source: Viscous gels on the west wall in the stone chamber of the Kitora Tumulus, Asuka Village, Nara Pref., Japan [11960].
Phylogeny: 16S rRNA gene (LC150649), gyrB (LC150732) [11960].
Taxonomy: Identification [11960].
BacDive ID: 163662.
Publication(s) using this strain [C17567].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Imposed
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
28866 <-- K. Okada and J. Sugiyama; Tokyo Natl. Res. Inst. for Cult. Prop. and TechnoSuruga Lab. Co., Ltd., Japan; K9703-3-1b.
Accessioned in 2016.
Bacillus simplex.
Medium: 475;  Temperature: 28°C; Rehydration fluid: 656.   more information +
Source: Whitish viscous gels on the stone of the east wall in the stone chamber of the Kitora Tumulus, Asuka Village, Nara Pref., Japan [11960].
Phylogeny: 16S rRNA gene (LC150659), gyrB (LC150734) [11960].
Taxonomy: Identification [11960].
BacDive ID: 163672.
Publication(s) using this strain [C17567].
 Related information on delivery / use of the strain
Biosafety level 1 -
Terms and conditions Imposed
Export control (1) No -
Distribution control in Japan (2) No -
Genetically modified microorganism No -
Technical information - -
Additional information - -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Overseas A (Freeze-dried or L-dried culture) or C (Actively growing culture on request)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.
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