Medium data

Search for medium no. = [988].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

988   MPOB MEDIUM

Na2HPO4  0.53    g
KH2PO4  0.41    g
NH4Cl  0.30    g
CaCl2·2H2O  0.11    g
MgCl2·6H2O  0.10    g
NaCl  0.30    g
Trace element solution SL-10 (see Medium No. 433)  1.0     ml
Selenite-tungstate solution (see Medium No. 554)  1.0     ml
Yeast extract (BD-Difco)  0.4     g
Resazurin  0.5     mg
NaHCO3  4.0     g
Trace vitamins (see Medium No. 197)  10.0     ml
Glycerol  2.3     g
Na2S·9H2O  0.5     g
Distilled water  1.0     L
Mix ingredients except Trace vitamins, NaHCO3 and Na2S·9H2O. Separately autoclave NaHCO3 and Na2S·9H2O (4 and 5% solutions, respectively) under a N2-CO2 atmosphere. Anaerobically and aseptically add the NaHCO3 and Na2S·9H2O solutions, and filter-sterilized Trace vitamins to the medium. Check final pH to be 7.0 - 7.2. Pressurize the inoculated bottles to 70 kPa N2-CO2 (4:1, v/v).