Medium data

Search for medium no. = [988].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

988 MPOB MEDIUM

Na₂HPO₄	`0.53`	g
KH₂PO₄	`0.41`	g
NH₄Cl	`0.30`	g
CaCl₂·2H₂O	`0.11`	g
MgCl₂·6H₂O	`0.10`	g
NaCl	`0.30`	g
Trace element solution SL-10 (see Medium No. 433)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 554)	`1.0`	ml
Yeast extract (BD-Difco)	`0.4`	g
Resazurin	`0.5`	mg
NaHCO₃	`4.0`	g
Trace vitamins (see Medium No. 197)	`10.0`	ml
Glycerol	`2.3`	g
Na₂S·9H₂O	`0.5`	g
Distilled water	`1.0`	L

Mix ingredients except Trace vitamins, NaHCO₃ and Na₂S·9H₂O. Separately autoclave NaHCO₃ and Na₂S·9H₂O (4 and 5% solutions, respectively) under a N₂-CO₂ atmosphere. Anaerobically and aseptically add the NaHCO₃ and Na₂S·9H₂O solutions, and filter-sterilized Trace vitamins to the medium. Check final pH to be 7.0 - 7.2. Pressurize the inoculated bottles to 70 kPa N₂-CO₂ (4:1, v/v).