Medium data

Search for medium no. = [433].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

433   PSEUDOMONAS HALOPHILA MEDIUM

Solution A:
NaCl  46.8     g
MgSO4·7H2O  39.4     g
NH4Cl  1.0     g
Glycerol  5.0     g
Trace element (see below)  1.0     ml
Vitamin solution (filter-sterilized, see below)  10.0     ml
Distilled water  890.0     ml
Solution B:
KH2PO4  1.0     g
Distilled water  100.0     ml
Trace element solution SL-10:
HCl (25%, 7.7 M)  10.0     ml
FeCl2·4H2O  1.5     g
ZnCl2  70.0     mg
MnCl2·4H2O  100.0     mg
H3BO3  6.0     mg
CoCl2·6H2O  190.0     mg
CuCl2·2H2O  2.0     mg
NiCl2·6H2O  24.0     mg
Na2MoO4·2H2O  36.0     mg
Distilled water  990.0     ml
Dissolve FeCl2·4H2O first in HCl. Then dilute in distilled water and add the remaining salts.
Vitamin solution:
Biotin  2.0     mg
Folic acid  2.0     mg
Pyridoxine·HCl  10.0     mg
Thiamine·HCl·2H2O  5.0     mg
Riboflavin  5.0     mg
Nicotinic acid  5.0     mg
Calcium pantothenate  5.0     mg
p-Aminobenzoic acid  1.0     mg
Vitamin B12  0.01    mg
Distilled water  1.0     L
Autoclave Solutions A and B separately and combine after cooling. Filter-sterilize vitamin solution and add to the autoclaved medium.
Adjust pH to 7.0.