Medium data

Search for medium no. = [554].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

554   TRICHLOROBACTER MEDIA (TTFA)

Sodium fumarate (adjust pH to 7.0)  1.16    g
KH2PO4 (dissolve first, adjust pH to 7.0)  0.2     g
NH4Cl  0.3     g
NaCl  1.0     g
MgCl2·6H2O  0.5     g
KCl  0.3     g
CaCl2·2H2O  0.015   g
Sodium acetate  0.68    g
SL10 elements (see below)  1.0     ml
Selenite-tungstate solution (see below)  1.0     ml
Distilled water  1.0     L
SL10 elements:
FeCl2·4H2O  1.5     g
CoCl2·6H2O  0.190   g
MnCl2·4H2O  0.100   g
ZnCl2  0.070   g
Na2MoO4·2H2O  0.036   g
NiCl2·6H2O  0.024   g
CuCl2·2H2O  0.002   g
Distilled water  1.0     L
Selenite-tungstate solution:
NaOH  0.5     g
Na2SeO3·5H2O  0.003   g
Na2WO4·2H2O  0.004   g
Distilled water  1.0     L


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