Medium data

Search for medium no. = [731].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

731 SPOROMUSA MEDIUM

Solution A:

NH₄Cl	`0.5`	g
MgSO₄·7H₂O	`0.5`	g
CaCl₂·2H₂O	`0.25`	g
NaCl	`2.25`	g
Trace vitamins (see Medium No. 197)	`10.0`	ml
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Se/W solution (see Medium No. 317)	`1.0`	ml
Betaine	`6.7`	g
Yeast extract	`2.0`	g
Casitone (BD-Difco)	`2.0`	g
Resazurin	`1.0`	mg
Distilled water	`850.0`	ml

Autoclave under a N₂ gas atmosphere.
Solution B:

K₂HPO₄	`0.348`	g
KH₂PO₄	`0.227`	g
Distilled water	`100.0`	ml

Autoclave under a N₂ gas atmosphere.
Solution C:

NaHCO₃	`4.0`	g
Distilled water	`50.0`	ml

Filter-sterilize and store under a N₂-CO₂ (4:1, v/v) atmosphere.
To complete the medium, combine Solutions A, B and C, and replace the gas phase with a N₂-CO₂ (4:1, v/v) gas mixture. Finally, add the following solutions from anaerobic stocks:

3% L-Cysteine·HCl·H₂O solution	`10.0`	ml
3% Na₂S·9H₂O solution	`10.0`	ml

Adjust pH of the medium to 7.0, if necessary.