Medium data

Search for medium no. = [731].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

731   SPOROMUSA MEDIUM

Solution A:
NH4Cl  0.5     g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.25    g
NaCl  2.25    g
Trace vitamins (see Medium No. 197)  10.0     ml
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Se/W solution (see Medium No. 317)  1.0     ml
Betaine  6.7     g
Yeast extract  2.0     g
Casitone (BD-Difco)  2.0     g
Resazurin  1.0     mg
Distilled water  850.0     ml
Autoclave under a N2 gas atmosphere.
Solution B:
K2HPO4  0.348   g
KH2PO4  0.227   g
Distilled water  100.0     ml
Autoclave under a N2 gas atmosphere.
Solution C:
NaHCO3  4.0     g
Distilled water  50.0     ml
Filter-sterilize and store under a N2-CO2 (4:1, v/v) atmosphere.
To complete the medium, combine Solutions A, B and C, and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Finally, add the following solutions from anaerobic stocks:
3% L-Cysteine·HCl·H2O solution  10.0     ml
3% Na2S·9H2O solution  10.0     ml
Adjust pH of the medium to 7.0, if necessary.