Medium data

Search for medium no. = [317].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

317   

Glucose  2.2     g
Yeast extract (BD-Difco)  2.3     g
KH2PO4  0.14    g
MgCl2·6H2O  0.2     g
CaCl2·2H2O  0.15    g
NH4Cl  0.54    g
NaHCO3  2.5     g
NaS2·9H2O  0.3     g
L-Cysteine·HCl·H2O  0.3     g
Trace vitamins (see Medium No. 197)  2.0     ml
Trace element solution (see below)  1.0     ml
Se/W solution (see below)  1.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Trace element solution:
Nitrilotriacetic acid  12.8     g
FeCl3·6H2O  1.35    g
MnCl2·4H2O  0.1     g
CoCl2·6H2O  0.024   g
CaCl2·2H2O  0.1     g
ZnCl2  0.1     g
CuCl2·2H2O  0.025   g
H3BO3  0.01    g
Na2MoO4·2H2O  0.024   g
NiCl2·6H2O  0.12    g
Distilled water  1.0     L
First dissolve nitrilotriacetic acid in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then, add the remaining salts and fill up to 1.0 L. Readjust pH to 7.0 with KOH.
Se/W solution:
Na2SeO3  4.0     mg
Na2WO4·2H2O  4.0     mg
Distilled water  1.0     L



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