Medium data

Search for medium no. = [187].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

187   DESULFUROCOCCUS AMYLOLYTICUS MEDIUM

NH4Cl  0.33    g
KH2PO4  0.33    g
KCl  0.33    g
CaCl2·2H2O  0.44    g
MgCl2·6H2O  0.7     g
NaCl  0.5     g
NaHCO3  0.8     g
FeCl2 solution (see below)  1.0     ml
Trace element solution (see below)  1.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Yeast extract (BD-Difco)  0.2     g
Bacto peptone (BD-Difco)  5.0     g
Sulfur (powder)  10.0     g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Distilled water  990.0     ml
Mix ingredients, except yeast extract, peptone, vitamins, sulfur and Na2S·9H2O, and autoclave under a N2-CO2 (80:20) atmosphere. Separately autoclave yeast extract and peptone as 10% solutions and Na2S·9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize NaHCO3 as a 8% solution and vitamin solution while gassing with N2. Aseptically and anaerobically add yeast extract, peptone, NaHCO3 and vitamin solutions, and sulfur to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S·9H2O solution, and adjust pH to 6.2-6.4 with sterile 1 N H2SO4.
FeCl2 solution:
25% HCl (7.7 M)  10.0     ml
FeCl2·4H2O  1.5     g
Distilled water  990.0     ml
Trace element solution:
ZnCl2  70.0     mg
MnCl2·4H2O  100.0     mg
H3BO3  6.0     mg
CoCl2·6H2O  190.0     mg
CuCl2·2H2O  2.0     mg
NiCl2·6H2O  24.0     mg
Na2MoO4·2H2O  36.0     mg
Distilled water  1.0     L