Medium data
Search for medium no. = [187].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
187 DESULFUROCOCCUS AMYLOLYTICUS MEDIUM
| NH4Cl | 0.33 | g |
| KH2PO4 | 0.33 | g |
| KCl | 0.33 | g |
| CaCl2·2H2O | 0.44 | g |
| MgCl2·6H2O | 0.7 | g |
| NaCl | 0.5 | g |
| NaHCO3 | 0.8 | g |
| FeCl2 solution (see below) | 1.0 | ml |
| Trace element solution (see below) | 1.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Yeast extract (BD-Difco) | 0.2 | g |
| Bacto peptone (BD-Difco) | 5.0 | g |
| Sulfur (powder) | 10.0 | g |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 990.0 | ml |
Mix ingredients, except yeast extract, peptone, vitamins, sulfur and Na2S·9H2O, and autoclave under a N2-CO2 (80:20) atmosphere. Separately autoclave yeast extract and peptone as 10% solutions and Na2S·9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize NaHCO3 as an 8% solution and vitamin solution while gassing with N2. Aseptically and anaerobically add yeast extract, peptone, NaHCO3 and vitamin solutions, and sulfur to the medium under a N2-CO2 (80:20) atmosphere. Prior to inoculation, add the sterile Na2S·9H2O solution, and adjust pH to 6.2-6.4 with sterile 1 N H2SO4.
FeCl2 solution:
| 25% HCl (7.7 M) | 10.0 | ml |
| FeCl2·4H2O | 1.5 | g |
| Distilled water | 990.0 | ml |
Trace element solution:
| ZnCl2 | 70.0 | mg |
| MnCl2·4H2O | 100.0 | mg |
| H3BO3 | 6.0 | mg |
| CoCl2·6H2O | 190.0 | mg |
| CuCl2·2H2O | 2.0 | mg |
| NiCl2·6H2O | 24.0 | mg |
| Na2MoO4·2H2O | 36.0 | mg |
| Distilled water | 1.0 | L |
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