Medium data

Search for medium no. = [1425].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1425 SPOROMUSA MEDIUM-2

Solution A:

NH₄Cl	`0.5`	g
MgSO₄·7H₂O	`0.5`	g
CaCl₂·2H₂O	`0.25`	g
NaCl	`2.25`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Se/W solution (see Medium No. 317)	`1.0`	ml
0.1% FeSO₄·7H₂O solution (in 0.1 N H₂SO₄)	`2.0`	ml
Yeast extract	`2.0`	g
Casitone (BD-Difco)	`2.0`	g
Resazurin	`0.5`	mg
Distilled water	`860.0`	ml

Autoclave under a N₂ gas atmosphere.
Solution B:

K₂HPO₄	`0.35`	g
KH₂PO₄	`0.23`	g
Distilled water	`100.0`	ml

Autoclave under a N₂ gas atmosphere.
After autoclaving, combine solutions A and B, then add the following solutions (filtersterilized):

Trace vitamins (see Medium No. 197)	`10.0`	ml
8% NaHCO₃ solution	`30.0`	ml

To complete the medium, aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a H₂-CO₂ (80:20, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution (freshly prepared and filter-sterilized):

0.1 M Dithiothreitol solution 10.0 ml

Adjust pH to 7.0, if necessary.