Medium data

Search for medium no. = [1425].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1425   SPOROMUSA MEDIUM-2

Solution A:
NH4Cl  0.5     g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.25    g
NaCl  2.25    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Se/W solution (see Medium No. 317)  1.0     ml
0.1% FeSO4·7H2O solution (in 0.1 N H2SO4)  2.0     ml
Yeast extract  2.0     g
Casitone (BD-Difco)  2.0     g
Resazurin  0.5     mg
Distilled water  860.0     ml
Autoclave under a N2 gas atmosphere.
Solution B:
K2HPO4  0.35    g
KH2PO4  0.23    g
Distilled water  100.0     ml
Autoclave under a N2 gas atmosphere.
After autoclaving, combine solutions A and B, then add the following solutions (filtersterilized):
Trace vitamins (see Medium No. 197)  10.0     ml
8% NaHCO3 solution  30.0     ml
To complete the medium, aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a H2-CO2 (80:20, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution (freshly prepared and filter-sterilized):
0.1 M Dithiothreitol solution  10.0     ml
Adjust pH to 7.0, if necessary.



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