Medium data

Search for medium no. = [1413].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1413 TEPIDIBACILLUS MARIANAENSIS MEDIUM

NaCl	`26.0`	g
MgCl₂·6H₂O	`5.0`	g
CaCl₂·2H₂O	`1.4`	g
Na₂SO₄	`1.3`	g
NH₄Cl	`0.3`	g
KH₂PO₄	`0.1`	g
KCl	`0.5`	g
SL-7 trace element solution (see Medium No. 536)	`1.0`	ml
Casamino acids	`1.0`	g
L-Cysteine·HCl·H₂O	`0.031`	g
Distilled water	`940.0`	ml

Mix components thoroughly, and autoclave under a N₂-CO₂ (80:20, v/v) gas mixture. After cooling, aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`30.0`	ml
Trace vitamins* (see Medium No. 197)	`1.0`	ml
Thiamine solution* (see Medium No. 403)	`1.0`	ml
Vitamin B₁₂ solution* (see Medium No. 403)	`1.0`	ml
1.0 M Sodium pyruvate solution	`10.0`	ml
Iron(III) citrate solution (see Medium No. 1014)	`20.0`	ml