Medium data

Search for medium no. = [536].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

536   PHE91 MEDIUM

Basal salt solution (see below)  969.0     ml
SL-7 trace element solution (see below)  1.0     ml
Vitamin solution (see below)  1.0     ml
Vitamin B12 solution (see Medium No. 403)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
CaCl2·2H2O solution (68 mM)  1.0     ml
K2HPO4 solution (609 mM)  10.0     ml
Resazurin solution (0.02%)  1.0     ml
Dithionite solution (0.2 M)  5.0     ml
Pyruvate solution (1 M)  10.0     ml
Autoclave the basal solution under a N2 gas atmosphere. Separately filter-sterilize the other solutions and add to the medium anaerobically and aseptically. Prepare dithionite solution just before use.
Basal solution:
NH4H2PO4  0.46    g
MgSO4·7H2O  0.49    g
Distilled water  969.0     ml
SL-7 trace element solution:
25% HCl (7.7 M)  10.0     ml
FeCl2·4H2O  1.5     g
CoCl2·6H2O  190.0     mg
MnCl2·4H2O  100.0     mg
ZnCl2  70.0     mg
H3BO3  62.0     mg
Na2MoO4·2H2O  36.0     mg
NiCl2·6H2O  24.0     mg
CuCl2·2H2O  17.0     mg
Distilled water  1.0     L
Initially, dissolve FeCl2·4H2O in 25% HCl, then add distilled water followed by the other components.
Vitamin solution:
Biotin  2.5     mg
Nicotinic acid  25.0     mg
Thiamine·HCl  12.5     mg
p-Aminobenzoic acid  12.5     mg
DL-Calcium pantothenate  6.5     mg
Pyridoxine·HCl  62.5     mg
Distilled water  1.0     L
Store in the dark at 4C.



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