Medium data
Search for medium no. = [1014].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1014 NASU IRON REDUCER MEDIUM
| (NH4)2SO4 | 0.2 | g |
| KH2PO4 | 3.0 | g |
| MgSO4·7H2O | 0.5 | g |
| CaCl2·2H2O | 0.25 | g |
| Yeast extract | 0.5 | g |
| Modified Allen's trace metal solution (see below) | 1.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
| Iron(III) citrate solution (see below) | 100.0 | ml |
| Distilled water | 900.0 | ml |
Mix components thoroughly and adjust pH to 6.0. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N2 atmosphere):
| 25 mM L-Cysteine·HCI solution | 10.0 | ml |
| 130 mM FeCl2 solution | 10.0 | ml |
Modified Allen's trace metal solution:
| MnCl2·4H2O | 1.8 | g |
| Na2B4O7·10H2O | 4.5 | g |
| ZnSO4·7H2O | 0.22 | g |
| CuCl2·2H2O | 0.05 | g |
| Na2MoO4·2H2O | 0.03 | g |
| VOSO4·xH2O | 0.03 | g |
| CoSO4·7H2O | 0.01 | g |
| Distilled water | 1.0 | L |
Iron(III) citrate solution:
Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved. Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution. Fill up to 100 ml with distilled water.
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