Medium data

Search for medium no. = [1014].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


(NH4)2SO4  0.2     g
KH2PO4  3.0     g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.25    g
Yeast extract  0.5     g
Modified Allen's trace metal solution (see below)  1.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
Iron(III) citrate solution (see below)  100.0     ml
Distilled water  900.0     ml
Mix components thoroughly and adjust pH to 6.0. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N2 atmosphere):
25 mM L-Cysteine·HCI solution  10.0     ml
130 mM FeCl2 solution  10.0     ml
Modified Allen's trace metal solution:
MnCl2·4H2O  1.8     g
Na2B4O7·10H2O  4.5     g
ZnSO4·7H2O  0.22    g
CuCl2·2H2O  0.05    g
Na2MoO4·2H2O  0.03    g
VOSO4·xH2O  0.03    g
CoSO4·7H2O  0.01    g
Distilled water  1.0     L
Iron(III) citrate solution:
Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved. Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution. Fill up to 100 ml with distilled water.

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