Medium data
Search for medium no. = [1333].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1333 DESULFURIVIBRIO AMeS2 MEDIUM
| Na2CO3 | 22.0 | g |
| NaHCO3 | 8.0 | g |
| NaCl | 6.0 | g |
| K2HPO4 | 1.0 | g |
| Sulfur | 5.0 | g |
Add components, except sulfur, to distilled water and bring volume to 1.0 L. Autoclave in closed bottles. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
| 1 M MgCl2 solution | 1.0 | ml |
| 1 M NH4Cl solution | 4.0 | ml |
| Trace element solution (see Medium No. 1079) | 1.0 | ml |
| Se/W solution* (see Medium No. 852) | 1.0 | ml |
| Trace vitamins* (see Medium No. 197) | 1.0 | ml |
Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically distribute the medium and sterilized sulfur into culture vessels (e.g., 10 ml medium and 0.05 g sulfur in Hungate tubes) under a N2 gas steam and seal with butyl rubber stoppers.
If the inoculum does not contain sulfide/polysulfide, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
| 5% Na2S·9H2O solution | 5.0 | ml |
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