Medium data

Search for medium no. = [1284].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Base salt medium (see Medium No. 1207)  1.0     L
Autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized):
1 M MgSO4 solution  1.0     ml
Trace element solution (see Medium No. 1079)  1.0     ml
Selenite-tungstate solution* (see Medium No. 431)  1.0     ml
Trace vitamins* (see Medium No. 197)  5.0     ml
To complete the medium, aseptically and anaerobically distribute the medium into culture vessels containing steam-sterilized sulfur (see below, approximately 5.0 g/L) under an argon gas stream, and seal with butyl rubber stoppers. Then, add per liter the following solutions (autoclaved or *filter-sterilized):
10% Yeast extract solution  5.0     ml
5.0 M Sodium formate solution*  10.0     ml
5% Na2S·9H2O solution  2.5     ml
Sterilization of sulfur:
Steam sulfur for 3 hr, or autoclave at 105C for 1 hr, on each of 3 successive days.

Copyright © 2024 Microbe Division (JCM) - All Rights Reserved