Medium data
Search for medium no. = [1192].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1192 GEOTHERMOBACTER MEDIUM
| NaCl | 15.0 | g |
| MgCl2·6H2O | 4.0 | g |
| KCl | 0.33 | g |
| CaCl2·2H2O | 0.33 | g |
| (NH4)2SO4 | 0.5 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Selenite-tungstate solution (see Medium No. 431) | 1.0 | ml |
| Distilled water | 840.0 | ml |
Mix components thoroughly, distribute the medium in a culture bottle under a H2-CO2 (4:1, v/v) gas stream, seal with a butyl rubber stopper and autoclave. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
| Iron(III) citrate solution (see Medium No. 1014) | 100.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
| 8% NaHCO3 solution* | 30.0 | ml |
| 0.1 M KH2PO4 solution | 20.0 | ml |
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