Medium data

Search for medium no. = [1053].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1053 DEFERRISOMA PALEOCHRORII MEDIUM

NaCl	`15.0`	g
MgCl₂·6H₂O	`4.0`	g
KCl	`0.33`	g
CaCl₂·2H₂O	`0.33`	g
(NH₄)₂SO₄	`0.5`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`0.5`	ml
Distilled water	`860.0`	ml

Mix components thoroughly, adjust pH to 6.0 with 1 N HCl and autoclave under a H₂-CO₂ (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):

3.3% KH₂PO₄ solution	`10.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml
8% NaHCO₃ solution*	`10.0`	ml
20% (w/v) MES (pH 6.0) solution	`10.0`	ml
Iron(III)citrate solution (see Medium No. 1014)	`100.0`	ml

Readjust pH to 6.0 to 6.3, if necessary.