Medium data

Search for medium no. = [1053].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1053   DEFERRISOMA PALEOCHRORII MEDIUM

NaCl  15.0     g
MgCl2·6H2O  4.0     g
KCl  0.33    g
CaCl2·2H2O  0.33    g
(NH4)2SO4  0.5     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  0.5     ml
Distilled water  860.0     ml
Mix components thoroughly, adjust pH to 6.0 with 1 N HCl and autoclave under a H2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
3.3% KH2PO4 solution  10.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
8% NaHCO3 solution*  10.0     ml
20% (w/v) MES (pH 6.0) solution  10.0     ml
Iron(III)citrate solution (see Medium No. 1014)  100.0     ml
Readjust pH to 6.0 to 6.3, if necessary.