Medium data

Search for medium no. = [998].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

998 DESULFOSPOROSINUS ACIDODURANS MEDIUM

Na₂SO₄	`1.42`	g
Na₂HPO₄·2H₂O	`0.53`	g
KH₂PO₄	`0.41`	g
NH₄Cl	`0.3`	g
CaCl₂·2H₂O	`0.11`	g
MgCl₂·6H₂O	`0.1`	g
NaCl	`0.3`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Yeast extract (BD-Difco)	`0.2`	g
Resazurin	`0.5`	mg
Distilled water	`1.0`	L

Mix components thoroughly, bring to a boil for a few seconds and cool down under a N₂-CO₂ (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5.0 ml in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized and stored under a N₂ atmosphere):

5% (w/v) glycerin solution	`10.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml
5% L-Cysteine·HCl·H₂O solution	`10.0`	ml

Comment: For JCM 32882 and JCM 32883, add per liter 10.0 g iron powder in the medium. Then, adjust final pH of the medium to 2.5.