Medium data

Search for medium no. = [994].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

994   MBM MEDIUM 2

NaCl  80.0     g
MgSO4·7H2O  6.0     g
KCl  1.5     g
NH4Cl  1.0     g
CaCl2·2H2O  0.4     g
K2HPO4  0.4     g
Trace minerals (see Medium No. 151)  10.0     ml
Sodium acetate·3H2O  1.6     g
Resazurin  1.0     mg
Distilled water  900.0     ml
Mix components thoroughly. Bring to a boil for a few seconds, cool down under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized):
1 M Disodium fumarate solution  40.0     ml
8% NaHCO3 solution*  50.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
Aseptically distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 gas atmosphere):
5% L-Cysteine·HCl·H2O solution  6.0     ml
5% Na2S·9H2O solution  6.0     ml
Readjust pH to 7.5, if necessary.

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