Medium data
Search for medium no. = [994].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
994 MBM MEDIUM 2
| NaCl | 80.0 | g |
| MgSO4·7H2O | 6.0 | g |
| KCl | 1.5 | g |
| NH4Cl | 1.0 | g |
| CaCl2·2H2O | 0.4 | g |
| K2HPO4 | 0.4 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Sodium acetate·3H2O | 1.6 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 900.0 | ml |
Mix components thoroughly. Bring to a boil for a few seconds, cool down under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized):
| 1 M Disodium fumarate solution | 40.0 | ml |
| 8% NaHCO3 solution* | 50.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
Aseptically distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 gas atmosphere):
| 5% L-Cysteine·HCl·H2O solution | 6.0 | ml |
| 5% Na2S·9H2O solution | 6.0 | ml |
Readjust pH to 7.5, if necessary.
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