Medium data
Search for medium no. = [151].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
151 PYROCOCCUS MEDIUM
| Salt base solution (see below) | 1.0 | L |
| Trace minerals (see below) | 10.0 | ml |
| Citric acid | 5.0 | mg |
| Yeast extract (BD-Difco) | 1.0 | g |
| Bacto peptone (BD-Difco) | 5.0 | g |
| Sulfur (powder) | 30.0 | g |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
Mix ingredients, except sulfur and Na2S·9H2O, and adjust pH to 6.5 with H2SO4. Sterilize by filtration under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S·9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add sterile sulfur to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution, and readjust pH to 6.5, if necessary.
Salt base solution:
| NaCl | 13.85 | g |
| MgSO4·7H2O | 3.5 | g |
| MgCl2·6H2O | 2.75 | g |
| KCl | 0.325 | g |
| NaBr | 0.05 | g |
| H3BO3 | 15.0 | mg |
| SrCl2·6H2O | 7.5 | mg |
| KI | 0.05 | mg |
| CaCl2 | 0.75 | g |
| KH2PO4 | 0.5 | g |
| (NH4)2Ni(SO4)2·6H2O | 2.0 | mg |
| Distilled water | 1.0 | L |
Trace minerals:
| Nitrilotriacetic acid | 1.5 | g |
| MgSO4·7H2O | 3.0 | g |
| MnSO4·xH2O | 0.5 | g |
| NaCl | 1.0 | g |
| FeSO4·7H2O | 0.1 | g |
| CoSO4·7H2O | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| ZnSO4·7H2O | 0.1 | g |
| CuSO4·5H2O | 0.01 | g |
| AlK(SO4)2 | 0.01 | g |
| H3BO3 | 0.01 | g |
| Na2MoO4·2H2O | 0.01 | g |
| Distilled water | 1.0 | L |
Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
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