Medium data

Search for medium no. = [992].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

992 DILUTED NITRATE MINERAL MEDIUM

MgSO₄·7H₂O	`0.2`	g
KNO₃	`0.2`	g
CaCl₂·2H₂O	`0.03`	g
Modified SL-4 trace element solution (see below)	`1.0`	ml
Chelated iron solution (see Medium No. 828)	`10.0`	ml
Distilled water	`990.0`	ml

Mix components thoroughly. For preparation of agar medium, add 15.0 g/L agar. Autoclave and, after cooling to 60C, add the following solutions (filter-sterilized):

0.2 M Na₂HPO₄ solution	`5.0`	ml
0.2 M KH₂PO₄ solution	`5.0`	ml

pH of the medium should be around 6.8. Cultivate under an air containing methane in gastight flasks or jars leaving ≥80% gas phase by volume (20% methane for liquid cultures; 50% methane for agar plate cultures).
Modified SL-4 trace element solution:

EDTA·2Na	`0.5`	g
FeSO₄·7H₂O	`0.2`	g
CuSO₄·5H₂O	`0.25`	g
SL-6 trace element solution (see Medium No. 167)	`100.0`	ml
Distilled water	`900.0`	ml