Medium data
Search for medium no. = [828].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
828 MODIFIED NMS MEDIUM
| MgSO4·7H2O | 1.0 | g |
| CaCl2·2H2O | 0.2 | g |
| KNO3 | 1.0 | g |
| SL-4 trace element solution (see Medium No. 340) | 5.0 | ml |
| Chelated iron solution (see below) | 10.0 | ml |
| Distilled water | 900.0 | ml |
Mix components and autoclave. After cooling, add the following solutions (autoclaved) to the medium:
| Phosphate buffer solution (see below) | 100.0 | ml |
Adjust pH to 6.8, if necessary. Aseptically distribute the medium into sterile serum bottles (leave ≥ 80% gas phase by volume) and seal with rubber stoppers. Replace the gas phase with an air-methane gas mixture (75:25, v/v). Cultivate with shaking.
Chelated iron solution:
| FeCl3·6H2O | 0.027 | g |
| EDTA·2Na | 0.0372 | g |
| Distilled water | 100.0 | ml |
Phosphate buffer solution:
| KH2PO4 | 0.272 | g |
| Na2HPO4·2H2O | 0.717 | g |
| Distilled water | 100.0 | ml |
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