Medium data

Search for medium no. = [340].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

340 MBM MEDIUM

NaNO₃	`0.2`	g
KH₂PO₄	`0.2`	g
NH₄Cl	`0.2`	g
MgCl₂·6H₂O	`0.4`	g
KCl	`0.2`	g
CaCl₂·2H₂O	`0.1`	g
NaHCO₃	`1.0`	g
Distilled water	`1.0`	L

Mix components thoroughly and autoclave. After cooling, add the following solutions (filter-sterilized):

SL-4 trace element solution (see below)	`2.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml

Aseptically, distribute the medium into culture vessels (e. g. 20 ml in 120 ml serum bottles) under a stream of N₂-CO₂ (4:1, v/v), and seal with butyl rubber stoppers. Add O₂ to make 1% (v/v) volume in the gas phase. Prior to inoculation, add per liter the following solution (autoclaved and stored under a N₂ atmosphere):

5% Na₂S·9H₂O solution 10.0 ml

SL-4 trace element solution:

EDTA	`0.5`	g
FeSO₄·7H₂O	`0.2`	g
SL-6 trace element solution (see Medium No. 167)	`100.0`	ml
Distilled water	`900.0`	ml