Medium data

Search for medium no. = [167].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

167   

KH2PO4  1.0     g
KCl  1.0     g
NH4Cl  1.0     g
MgSO4·7H2O  0.24    g
CaSO4·2H2O  0.17    g
SL-6 trace element solution (see below)  1.0     ml
NaCl  200.0     g
Sodium glutamate  1.0     g
Yeast extract (BD-Difco)  5.0     g
Casamino acids (BD-Difco)  5.0     g
Na2CO3  5.0     g
Agar  20.0     g
Add components except Na2CO3 to distilled water and bring volume to 900 ml. Adjust pH to 6.5 and autoclave. Dissolve Na2CO3 in 100 ml distilled water and autoclave separately. Aseptically add the sterile Na2CO3 solution to the medium. Check final pH to be 9.0-9.5.
SL-6 trace element solution:
ZnSO4·7H2O  0.1     g
MnCl2·4H2O  0.03    g
H3BO3  0.3     g
CoCl2·6H2O  0.2     g
CuCl2·2H2O  0.01    g
NiCl2·6H2O  0.02    g
Na2MoO4·2H2O  0.03    g
Distilled water  1.0     L
Adjust pH to 3.6.