Medium data
Search for medium no. = [167].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
167
| KH2PO4 | 1.0 | g |
| KCl | 1.0 | g |
| NH4Cl | 1.0 | g |
| MgSO4·7H2O | 0.24 | g |
| CaSO4·2H2O | 0.17 | g |
| SL-6 trace element solution (see below) | 1.0 | ml |
| NaCl | 200.0 | g |
| Sodium glutamate | 1.0 | g |
| Yeast extract (BD-Difco) | 5.0 | g |
| Casamino acids (BD-Difco) | 5.0 | g |
| Na2CO3 | 5.0 | g |
| Agar | 20.0 | g |
Add components except Na2CO3 to distilled water and bring volume to 900 ml. Adjust pH to 6.5 and autoclave. Dissolve Na2CO3 in 100 ml distilled water and autoclave separately. Aseptically add the sterile Na2CO3 solution to the medium. Check final pH to be 9.0-9.5.
SL-6 trace element solution:
| ZnSO4·7H2O | 0.1 | g |
| MnCl2·4H2O | 0.03 | g |
| H3BO3 | 0.3 | g |
| CoCl2·6H2O | 0.2 | g |
| CuCl2·2H2O | 0.01 | g |
| NiCl2·6H2O | 0.02 | g |
| Na2MoO4·2H2O | 0.03 | g |
| Distilled water | 1.0 | L |
Adjust pH to 3.6.
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