Medium data

Search for medium no. = [990].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

990   MODIFIED MPOB MEDIUM

Na2HPO4  0.53    g
KH2PO4  0.41    g
NH4Cl  0.30    g
CaCl2·2H2O  0.11    g
MgCl2·6H2O  0.10    g
NaCl  0.30    g
Trace element solution SL-10 (see Medium No. 433)  1.0     ml
Yeast extract (BD-Difco)  0.4     g
Resazurin  0.5     mg
Trace vitamins (see Medium No. 197)  10.0     ml
Glucose  1.8     g
L-Cysteine·HCl·H2O  0.5     g
Distilled water  1.0     L
Prepare the medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. Mix ingredients except Trace vitamins. After autoclaving, anaerobically and aseptically add the filter-sterilized Trace vitamins to the medium. Check final pH to be 7.0 - 7.2. Pressurize the inoculated bottles to 70 kPa N2-CO2 (4:1, v/v).