Medium data

Search for medium no. = [990].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

990 MODIFIED MPOB MEDIUM

Na₂HPO₄	`0.53`	g
KH₂PO₄	`0.41`	g
NH₄Cl	`0.30`	g
CaCl₂·2H₂O	`0.11`	g
MgCl₂·6H₂O	`0.10`	g
NaCl	`0.30`	g
Trace element solution SL-10 (see Medium No. 433)	`1.0`	ml
Yeast extract (BD-Difco)	`0.4`	g
Resazurin	`0.5`	mg
Trace vitamins (see Medium No. 197)	`10.0`	ml
Glucose	`1.8`	g
L-Cysteine·HCl·H₂O	`0.5`	g
Distilled water	`1.0`	L

Prepare the medium anaerobically under a N₂-CO₂ (4:1, v/v) gas mixture. Mix ingredients except Trace vitamins. After autoclaving, anaerobically and aseptically add the filter-sterilized Trace vitamins to the medium. Check final pH to be 7.0 - 7.2. Pressurize the inoculated bottles to 70 kPa N₂-CO₂ (4:1, v/v).