Medium data
Search for medium no. = [983].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
983 ARTIFICIAL SEAWATER MEDIUM WITH PULLULAN
| NaCl | 80.0 | g |
| MgSO4·7H2O | 25.0 | g |
| CaCl2·2H2O | 4.5 | g |
| KCl | 1.5 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Selenite-tungstate solution (see Medium No. 431) | 1.0 | ml |
| Resazurin | 0.5 | mg |
| Pullulan | 0.5 | g |
| Distilled water | 1.0 | L |
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and stored under N2):
| 1% NH4Cl solution | 10.0 | ml |
| 1% KH2PO4 solution | 5.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
| 5% Na2CO3 solution | 20.0 | ml |
| 5% Na2S·9H2O | 6.0 | ml |
Adjust pH to 7.2 - 7.5 with a 5% Na2CO3 solution, if necessary.
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