Medium data

Search for medium no. = [983].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

983 ARTIFICIAL SEAWATER MEDIUM WITH PULLULAN

NaCl	`80.0`	g
MgSO₄·7H₂O	`25.0`	g
CaCl₂·2H₂O	`4.5`	g
KCl	`1.5`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
Resazurin	`0.5`	mg
Pullulan	`0.5`	g
Distilled water	`1.0`	L

Mix components thoroughly and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and stored under N₂):

1% NH₄Cl solution	`10.0`	ml
1% KH₂PO₄ solution	`5.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml
5% Na₂CO₃ solution	`20.0`	ml
5% Na₂S·9H₂O	`6.0`	ml

Adjust pH to 7.2 - 7.5 with a 5% Na₂CO₃ solution, if necessary.