Medium data

Search for medium no. = [983].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

983   ARTIFICIAL SEAWATER MEDIUM WITH PULLULAN

NaCl  80.0     g
MgSO4·7H2O  25.0     g
CaCl2·2H2O  4.5     g
KCl  1.5     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Resazurin  0.5     mg
Pullulan  0.5     g
Distilled water  1.0     L
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and stored under N2):
1% NH4Cl solution  10.0     ml
1% KH2PO4 solution  5.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
5% Na2CO3 solution  20.0     ml
5% Na2S·9H2O  6.0     ml
Adjust pH to 7.2 - 7.5 with a 5% Na2CO3 solution, if necessary.