Medium data

Search for medium no. = [971].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

971 1/2 SME MEDIUM FOR YA

Synthetic seawater (2 x, see Medium No. 590)	`250.0`	ml
KH₂PO₄	`0.5`	g
NH₄Cl	`0.3`	g
Sodium acetate trihydrate	`1.08`	g
Yeast extract	`0.2`	g
Modified Wolfe's minerals (10 x, see below)	`1.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
Resazurin	`1.0`	mg
Distilled water	`740.0`	ml

Mix components thoroughly, boil for a few seconds and cool down under a stream of a N₂-CO₂(4:1, v/v) gas mixture. After cooling, add the following components and adjust pH to 7.0:

NaHCO₃	`3.0`	g
5% Na₂S·9H₂O solution	`6.0`	ml

Dispense the medium into culture vessels anaerobically under the same gas mixture, seal with butyl rubber stoppers and autoclave.
Modified Wolfe's minerals (10 x):

MgSO₄·7H₂O	`3.0`	g
MnSO₄·xH₂O	`0.5`	g
NaCl	`1.0`	g
FeSO₄·7H₂O	`0.1`	g
CoSO₄·7H₂O	`0.18`	g
CaCl₂·2H₂O	`0.1`	g
ZnSO₄·7H₂O	`0.18`	g
CuSO₄·5H₂O	`0.01`	g
AlK(SO₄)₂·12H₂O	`0.018`	g
H₃BO₃	`0.01`	g
Na₂MoO₄·2H₂O	`0.01`	g
(NH₄)₂Ni(SO₄)₂·6H₂O	`0.28`	g
Na₂SeO₄	`0.01`	g
Na₂WO₄·2H₂O	`0.01`	g
Distilled water	`100.0`	ml

Adjust pH to 1.0 with 1 N H₂SO₄.