Medium data

Search for medium no. = [971].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

971   1/2 SME MEDIUM FOR YA

Synthetic seawater (2 x, see Medium No. 590)  250.0     ml
KH2PO4  0.5     g
NH4Cl  0.3     g
Sodium acetate trihydrate  1.08    g
Yeast extract  0.2     g
Modified Wolfe's minerals (10 x, see below)  1.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Resazurin  1.0     mg
Distilled water  740.0     ml
Mix components thoroughly, boil for a few seconds and cool down under a stream of a N2-CO2(4:1, v/v) gas mixture. After cooling, add the following components and adjust pH to 7.0:
NaHCO3  3.0     g
5% Na2S·9H2O solution  6.0     ml
Dispense the medium into culture vessels anaerobically under the same gas mixture, seal with butyl rubber stoppers and autoclave.
Modified Wolfe's minerals (10 x):
MgSO4·7H2O  3.0     g
MnSO4·xH2O  0.5     g
NaCl  1.0     g
FeSO4·7H2O  0.1     g
CoSO4·7H2O  0.18    g
CaCl2·2H2O  0.1     g
ZnSO4·7H2O  0.18    g
CuSO4·5H2O  0.01    g
AlK(SO4)2·12H2O  0.018   g
H3BO3  0.01    g
Na2MoO4·2H2O  0.01    g
(NH4)2Ni(SO4)2·6H2O  0.28    g
Na2SeO4  0.01    g
Na2WO4·2H2O  0.01    g
Distilled water  100.0     ml
Adjust pH to 1.0 with 1 N H2SO4.



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