Medium data

Search for medium no. = [590].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

590 A-MMX MEDIUM

Synthetic seawater (2 x, see Medium No. 244)	`125.0`	ml
CaCl₂·2H₂O	`0.19`	g
NaCl	`113.0`	g
KH₂PO₄	`0.5`	g
Yeast extract	`0.2`	g
Peptone	`0.2`	g
Resazurin	`1.0`	mg
Na₂S·9H₂O	`0.5`	g
Starch solution (see below)	`100.0`	ml
Distilled water	`775.0`	ml

Mix components except Na₂S·9H₂O and bubble with N₂ for 30 min. Distribute the medium into appropriate culture vessels (e.g., 10 ml medium in the Bellco anaerobic culture tube) under a N₂ atmosphere, seal with butyl rubber stoppers. Add Na₂S·9H₂O as 5% solution and autoclave. Adjust pH to 6.5 - 7.0, if necessary.
Starch solution:

Soluble starch	`5.0`	g
Distilled water	`100.0`	ml

Boil water, then, add starch and mix until dissolves thoroughly.