Medium data
Search for medium no. = [957].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
957 DESULFOHALOBIUM UTAHENSE MEDIUM
| NaCl | 100.0 | g |
| MgSO4·7H2O | 10.0 | g |
| KCl | 6.0 | g |
| CaCl2·2H2O | 0.4 | g |
| NH4Cl | 1.0 | g |
| KH2PO4 | 0.1 | g |
| Yeast extract | 0.5 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Resazurin | 0.5 | mg |
| Distilled water | 890.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. Aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized) to the medium:
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
| 8% NaHCO3 solution* | 50.0 | ml |
| 5% L-Sodium lactate solution | 50.0 | ml |
Finally, add per liter the following solution from an anaerobic stock:
| 5% Na2S·9H2O solution | 6.0 | ml |
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