Medium data

Search for medium no. = [957].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

957   DESULFOHALOBIUM UTAHENSE MEDIUM

NaCl  100.0     g
MgSO4·7H2O  10.0     g
KCl  6.0     g
CaCl2·2H2O  0.4     g
NH4Cl  1.0     g
KH2PO4  0.1     g
Yeast extract  0.5     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Resazurin  0.5     mg
Distilled water  890.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. Aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized) to the medium:
Trace vitamins* (see Medium No. 197)  10.0     ml
8% NaHCO3 solution*  50.0     ml
5% L-Sodium lactate solution  50.0     ml
Finally, add per liter the following solution from an anaerobic stock:
5% Na2S·9H2O solution  6.0     ml



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