Medium data

Search for medium no. = [935].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

935   FERVIDICOCCUS FONTIS MEDIUM

NH4Cl  0.33    g
KH2PO4  0.33    g
KCl  0.33    g
CaCl2·2H2O  0.44    g
MgCl2·6H2O  0.7     g
NaCl  0.5     g
NaHCO3  0.8     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly, adjust pH to 5.0 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
10% (w/v) Yeast extract (BD-Difco) solution  5.0     ml
10% (w/v) Trypticase peptone (BD-BBL) solution  20.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
5% (w/v) Na2S·9H2O solution  10.0     ml
Readjust pH to 5.5 - 6.0 with sterile 1 N H2SO4, if necessary.



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