Medium data

Search for medium no. = [908].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

908   HALORHODOSPIRA NEUTRIPHILA MEDIUM

KH2PO4  1.0     g
NH4Cl  0.5     g
NaCl  100.0     g
MgCl2·6H2O  2.0     g
MgSO4·7H2O  0.5     g
CaCl2·2H2O  0.05    g
Trace element solution SL-12 (see Medium No. 496)  1.0     ml
NaHCO3 (10%, w/v)  20.0     ml
Na2S·9H2O (10%, w/v)  7.5     ml
Seven vitamins solution (see Medium No. 699)  1.0     ml
0.5 M Sodium acetate solution  4.0     ml
0.5 M Sodium succinate solution  2.0     ml
5% Yeast extract solution  10.0     ml
Distilled water  1.0     L
Adjust pH to 7.0 with 1 M Na2CO3 or 1 M H2SO4. Mix ingredients, except NaHCO3, Na2S·9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution, and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Separately autoclave acetate solution, succinate solution and yeast extract solution. Filter-sterilize NaHCO3, Na2S·9H2O. Aseptically and anaerobically add NaHCO3, Na2S·9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution to the medium.



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