Medium data
Search for medium no. = [496].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
496 MODIFIED BIEBL AND PFENNIG'S MEDIUM
| KH2PO4 | 0.5 | g |
| CaCl2·2H2O | 0.25 | g |
| MgSO4·7H2O | 1.0 | g |
| NH4Cl | 0.6 | g |
| Yeast extract (BD-Difco) | 0.3 | g |
| NaCl | 20.0 | g |
| Sorbitol | 3.0 | g |
| Distilled water | 1.0 | L |
Add the following component after autoclaving:
| Trace element solution SL-12 (see below) | 1.0 | ml |
Trace element solution SL-12:
| EDTA·2Na | 3.00 | g |
| FeSO4·7H2O | 1.10 | g |
| CoCl2·6H2O | 190.00 | mg |
| MnCl2·4H2O | 50.00 | mg |
| ZnCl2 | 42.00 | mg |
| NiCl2·6H2O | 24.00 | mg |
| Na2MoO4·2H2O | 18.00 | mg |
| H3BO3 | 300.00 | mg |
| CuCl2·2H2O | 2.00 | mg |
| Distilled water | 1.0 | L |
Autoclave separately.
Adjust pH to 6.8.
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