Medium data

Search for medium no. = [496].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

496   MODIFIED BIEBL AND PFENNIG'S MEDIUM

KH2PO4  0.5     g
CaCl2·2H2O  0.25    g
MgSO4·7H2O  1.0     g
NH4Cl  0.6     g
Yeast extract (BD-Difco)  0.3     g
NaCl  20.0     g
Sorbitol  3.0     g
Distilled water  1.0     L
Add the following component after autoclaving:
Trace element solution SL-12 (see below)  1.0     ml
Trace element solution SL-12:
EDTA·2Na  3.00    g
FeSO4·7H2O  1.10    g
CoCl2·6H2O  190.00    mg
MnCl2·4H2O  50.00    mg
ZnCl2  42.00    mg
NiCl2·6H2O  24.00    mg
Na2MoO4·2H2O  18.00    mg
H3BO3  300.00    mg
CuCl2·2H2O  2.00    mg
Distilled water  1.0     L
Autoclave separately.
Adjust pH to 6.8.