Medium data
Search for medium no. = [699].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
699 MODIFIED THIOCAPSA MEDIUM
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.4 | g |
| KH2PO4 | 0.2 | g |
| KCl | 0.5 | g |
| NH4Cl | 0.125 | g |
| CaCl2·2H2O | 0.15 | g |
| Na2SO4 | 0.15 | g |
| NaNO2 | 0.069 | g |
| Modified trace element solution SL-12 (see below) | 1.0 | ml |
| Seven vitamins solution (see below) | 1.0 | ml |
| Distilled water | 950.0 | ml |
Add NaHCO3 buffer (see below) and adjust pH to 7.2 - 7.4, if necessary.
NaHCO3 buffer:
| NaHCO3 | 2.5 | g |
| Distilled water | 50.0 | ml |
Modified trace element solution SL-12:
| H3BO3 | 300.0 | mg |
| FeSO4·7H2O | 1.0 | g |
| CoCl2·6H2O | 190.0 | mg |
| MnCl2·4H2O | 100.0 | mg |
| ZnCl2 | 70.0 | mg |
| NiCl2·6H2O | 24.0 | mg |
| Na2MoO4·2H2O | 18.0 | mg |
| CuCl2·2H2O | 2.0 | mg |
| Distilled water | 1.0 | L |
Seven vitamins solution:
| Vitamin B12 | 10.0 | mg |
| p-Aminobenzoic acid | 10.0 | mg |
| D(+)-Biotin | 2.0 | mg |
| Nicotinic acid | 20.0 | mg |
| Calcium pantothenate | 5.0 | mg |
| Pyridoxine·HCl | 50.0 | mg |
| Thiamine·HCl·2H2O | 10.0 | mg |
| Distilled water | 200.0 | ml |
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