Medium data

Search for medium no. = [699].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

699   MODIFIED THIOCAPSA MEDIUM

NaCl  1.0     g
MgCl2·6H2O  0.4     g
KH2PO4  0.2     g
KCl  0.5     g
NH4Cl  0.125   g
CaCl2·2H2O  0.15    g
Na2SO4  0.15    g
NaNO2  0.069   g
Modified trace element solution SL-12 (see below)  1.0     ml
Seven vitamins solution (see below)  1.0     ml
Distilled water  950.0     ml
Add NaHCO3 buffer (see below) and adjust pH to 7.2 - 7.4, if necessary.
NaHCO3 buffer:
NaHCO3  2.5     g
Distilled water  50.0     ml
Modified trace element solution SL-12:
H3BO3  300.0     mg
FeSO4·7H2O  1.0     g
CoCl2·6H2O  190.0     mg
MnCl2·4H2O  100.0     mg
ZnCl2  70.0     mg
NiCl2·6H2O  24.0     mg
Na2MoO4·2H2O  18.0     mg
CuCl2·2H2O  2.0     mg
Distilled water  1.0     L
Seven vitamins solution:
Vitamin B12  10.0     mg
p-Aminobenzoic acid  10.0     mg
D(+)-Biotin  2.0     mg
Nicotinic acid  20.0     mg
Calcium pantothenate  5.0     mg
Pyridoxine·HCl  50.0     mg
Thiamine·HCl·2H2O  10.0     mg
Distilled water  200.0     ml