Medium data

Search for medium no. = [907].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

907 GEOBACTER MEDIUM

KH₂PO₄	`0.136`	g
NH₄Cl	`0.535`	g
MgCl₂·6H₂O	`0.204`	g
CaCl₂·2H₂O	`0.147`	g
Sodium acetate	`0.82`	g
Na₂SO₄	`0.142`	g
Trace element solution (see Medium No. 243)	`10.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
Se/W solution (see Medium No. 284)	`1.0`	ml
Distilled water	`910.0`	ml

Mix components thoroughly and autoclave under a N₂-CO₂ (80:20, v/v) gas mixture. After cooling, add aseptically and anaerobically the following solutions (autoclaved or *filter-sterilized) to the medium:

8% NaHCO₃ solution*	`30.0`	ml
1.0 M Sodium fumarate solution	`10.0`	ml
Iron(III) citrate solution (see Medium No. 1014)	`50.0`	ml

Adjust pH to 7.0-7.2, if necessary.
Comment: Iron(III) citrate solution can be replaced by Fe NTA solution (filter-sterilized, see Medium No. 1355).