Medium data

Search for medium no. = [907].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

907   GEOBACTER MEDIUM

KH2PO4  0.136   g
NH4Cl  0.535   g
MgCl2·6H2O  0.204   g
CaCl2·2H2O  0.147   g
Sodium acetate  0.82    g
Na2SO4  0.142   g
Trace element solution (see Medium No. 243)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Se/W solution (see Medium No. 284)  1.0     ml
Distilled water  910.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, add aseptically and anaerobically the following solutions (autoclaved or *filter-sterilized) to the medium:
8% NaHCO3 solution*  30.0     ml
1.0 M Sodium fumarate solution  10.0      ml
Iron(III) citrate solution (see Medium No. 1014)  50.0      ml
Adjust pH to 7.0-7.2, if necessary.
Comment: Iron(III) citrate solution can be replaced by Fe NTA solution (filter-sterilized, see Medium No. 1355).



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