Medium data
Search for medium no. = [889].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
889 METALLIBACTERIUM MEDIUM
| (NH4)2SO4 | 132.0 | mg |
| MgCl2·6H2O | 53.0 | mg |
| KH2PO4 | 27.0 | mg |
| CaCl2·2H2O | 147.0 | mg |
| VOSO4·xH2O | 0.5 | mg |
| Wolfe's mineral elixir (see Medium No. 470) | 1.0 | ml |
| FeSO4·7H2O | 10.0 | mg |
| Trypticase peptone (BD-BBL) | 1.4 | g |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Distilled water | 1.0 | L |
Mix components, except Trace vitamins, adjust pH to 5.5 and autoclave. After cooling, aseptically add filter-sterilized Trace vitamins solution.
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