Medium data
Search for medium no. = [470].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
470 VULCANIBACILLUS MEDIUM
| Sea salts (Sigma) | 30.0 | g |
| NaNO3 | 1.0 | g |
| Wolfe's mineral elixir (see below) | 2.0 | ml |
| VOSO4·xH2O | 0.05 | mg |
| Sodium pyruvate | 1.0 | g |
| Resazurin | 0.5 | mg |
| FeSO4·7H2O | 0.1 | g |
| NaHCO3 | 1.0 | g |
| Yeast extract | 0.5 | g |
| Trace vitamins (see Medium No. 197) | 20.0 | ml |
| Distilled water | 1.0 | L |
Dissolve ingredients except FeSO4·7H2O, NaHCO3, yeast extract and vitamins, and adjust pH to 7.0. Bring to a boil for 3 min, then cool to room temperature under a N2 gas stream. Dispense the medium under the same gas atmosphere into culture vessels, seal with butyl rubber stoppers and autoclave. Add sterile anaerobic stock solutions of FeSO4·7H2O (1%, in 0.1 N H2SO4), NaHCO3 (8%, under a N2·CO2 gas mixture), yeast extract (10%) and vitamins (filter-sterilized) to the medium. Adjust final pH of the medium to 7.0 - 7.2. Add 10 - 20 mg sodium dithionite per liter [e.g., from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized], if the medium is not completely reduced after inoculation.
Wolfe's mineral elixir:
| MgSO4·7H2O | 30.0 | g |
| MnSO4·xH2O | 5.0 | g |
| NaCl | 10.0 | g |
| FeSO4·7H2O | 1.0 | g |
| CoCl2·6H2O | 1.8 | g |
| CaCl2·2H2O | 1.0 | g |
| ZnSO4·7H2O | 1.8 | g |
| CuSO4·5H2O | 0.1 | g |
| KAl(SO4)2·12H2O | 0.18 | g |
| H3BO3 | 0.1 | g |
| Na2MoO4·2H2O | 0.1 | g |
| (NH4)2Ni(SO4)2·6H2O | 2.8 | g |
| Na2WO4·2H2O | 0.1 | g |
| Na2SeO4 | 0.1 | g |
| Distilled water | 1.0 | L |
First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.
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