Medium data

Search for medium no. = [866].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

866 DESULFOTOMACULUM BS MEDIUM

KH₂PO₄	`0.3`	g
K₂HPO₄	`0.3`	g
NH₄Cl	`1.0`	g
NaCl	`1.0`	g
Na₂SO₄	`3.0`	g
MgCl₂·6H₂O	`0.5`	g
CaCl₂·2H₂O	`0.1`	g
KCl	`0.1`	g
Trace metal solution (see Medium No. 294)	`1.0`	ml
Resazurin	`1.0`	mg
Distilled water	`940.0`	ml

Mix components thoroughly, adjust pH to 7.2 with KOH, distribute the medium into culture vessels (e.g., 9.4 ml in Balch tubes) under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add the following solutions (per 9.4 ml medium, autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`0.25`	ml
1 M Sodium pyruvate solution	`0.2`	ml
Trace vitamins* (see Medium No. 197)	`0.05`	ml
1% Sodium dithionite solution* (freshly prepared)	`0.1`	ml
5% Na₂S·9H₂O solution	`0.04`	ml