Medium data

Search for medium no. = [866].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

866   DESULFOTOMACULUM BS MEDIUM

KH2PO4  0.3     g
K2HPO4  0.3     g
NH4Cl  1.0     g
NaCl  1.0     g
Na2SO4  3.0     g
MgCl2·6H2O  0.5     g
CaCl2·2H2O  0.1     g
KCl  0.1     g
Trace metal solution (see Medium No. 294)  1.0     ml
Resazurin  1.0     mg
Distilled water  940.0     ml
Mix components thoroughly, adjust pH to 7.2 with KOH, distribute the medium into culture vessels (e.g., 9.4 ml in Balch tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add the following solutions (per 9.4 ml medium, autoclaved or *filter-sterilized):
8% NaHCO3 solution*  0.25    ml
1 M Sodium pyruvate solution  0.2     ml
Trace vitamins* (see Medium No. 197)  0.05    ml
1% Sodium dithionite solution* (freshly prepared)  0.1     ml
5% Na2S·9H2O solution  0.04    ml