Medium data

Search for medium no. = [294].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

294   HALORHABDUS MEDIUM

NaCl  270.0     g
NaBr  0.1     g
MgSO4·7H2O  20.0     g
KCl  5.0     g
NH4Cl  2.0     g
Tris·HCl  12.0     g
Yeast extract (BD-Difco)  1.0     g
Trace metal solution (see below)  2.0     ml
Glucose  2.0     g
KH2PO4  0.125   g
CaCl2·2H2O  0.05    g
FeCl2·4H2O  5.0     mg
MnCl2·4H2O  5.0     mg
Add components except yeast extract to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with NaOH and sterilize by filtration. Autoclave yeast extract as a 10% (w/v) solution and aseptically add to the mineral solution.
Trace metal solution:
HCl (32%)  10.0     ml
FeCl2·4H2O  2.0     g
CoCl2·6H2O  0.25    g
MnCl2·4H2O  0.1     g
ZnCl2  0.07    g
H3BO3  0.006   g
Na2MoO4·2H2O  0.04    g
NiCl2·6H2O  0.07    g
CuCl2·2H2O  0.002   g
AlCl3  0.025   g
Na2WO4·2H2O  0.006   g
Distilled water  1.0     L



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