Medium data
Search for medium no. = [294].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
294 HALORHABDUS MEDIUM
| NaCl | 270.0 | g |
| NaBr | 0.1 | g |
| MgSO4·7H2O | 20.0 | g |
| KCl | 5.0 | g |
| NH4Cl | 2.0 | g |
| Tris·HCl | 12.0 | g |
| Yeast extract (BD-Difco) | 1.0 | g |
| Trace metal solution (see below) | 2.0 | ml |
| Glucose | 2.0 | g |
| KH2PO4 | 0.125 | g |
| CaCl2·2H2O | 0.05 | g |
| FeCl2·4H2O | 5.0 | mg |
| MnCl2·4H2O | 5.0 | mg |
Add components except yeast extract to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with NaOH and sterilize by filtration. Autoclave yeast extract as a 10% (w/v) solution and aseptically add to the mineral solution.
Trace metal solution:
| HCl (32%) | 10.0 | ml |
| FeCl2·4H2O | 2.0 | g |
| CoCl2·6H2O | 0.25 | g |
| MnCl2·4H2O | 0.1 | g |
| ZnCl2 | 0.07 | g |
| H3BO3 | 0.006 | g |
| Na2MoO4·2H2O | 0.04 | g |
| NiCl2·6H2O | 0.07 | g |
| CuCl2·2H2O | 0.002 | g |
| AlCl3 | 0.025 | g |
| Na2WO4·2H2O | 0.006 | g |
| Distilled water | 1.0 | L |
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved