Medium data

Search for medium no. = [837].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

837 SYFAC MEDIUM

Sea salts (Sigma)	`35.0`	g
Wolfe's mineral elixir (see Medium No. 470)	`1.0`	ml
Yeast extract (BD-Difco)	`1.0`	g
Resazurin	`0.5`	mg
Distilled water	`925.0`	ml

Mix components thoroughly and autoclave under a N₂ atmosphere. Aseptically and anaerobically add the following solutions from sterile anaerobic stock solutions (autoclaved or *filter-sterilized):

1 M Sodium acetate solution	`20.0`	ml
1 M Sodium fumarate solution	`20.0`	ml
Trace vitamins (see Medium No. 197)*	`10.0`	ml
8% NaHCO₃ solution*	`25.0`	ml

Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of N₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally add per liter the following solution (autoclaved and stored under a N₂ atmosphere):

5% Na₂S·9H₂O solution 6.0 ml