Medium data

Search for medium no. = [825].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

825   KOKO MEDIUM

Tryptone (BD-Difco)  1.0     g
Peptone  1.0     g
Yeast extract  1.0     g
K2HPO4  1.6     g
NaH2PO4·2H2O  1.0     g
NH4Cl  0.5     g
MgSO4·7H2O  0.16    g
CaCl2·2H2O  0.06    g
SL-4 trace element solution (see Medium No. 340)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Resazurin  0.5     mg
Distilled water  930.0     ml
Mix components thoroughly and adjust pH to 7.0. Autoclave the medium under a N2 atmosphere. Add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
10% Glucose solution  50.0     ml
8% NaHCO3 solution*  1.25    ml
3% L-Cysteine·HCl·H2O solution  10.0     ml
3% Na2S·9H2O solution  10.0     ml



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