Medium data

Search for medium no. = [823].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

823   TEPIDIBACTER MESOPHILUS MEDIUM

NaCl  5.0     g
MgCl2·6H2O  4.0     g
KCl  0.34    g
NH4Cl  0.25    g
CaCl2·2H2O  0.11    g
K2HPO4  0.18    g
Fe(NH4)2(SO4)2·6H2O  2.0     mg
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Yeast extract  0.2     g
Casitone (BD-Difco)  2.0     g
Trace vitamins (see Medium No. 197)  10.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components and bring to a boil for 5-10 sec and cool down under a N2-CO2 (80:20, v/v) gas stream. Dispense the medium into culture vessels (e.g., 20 ml in 50 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks:
5% Na2S·9H2O solution  10.0     ml
8% NaHCO3 solution  5.0     ml
To prepare the stock solutions, autoclave 5% Na2S·9H2O solution under a N2 atmosphere, filter-sterilize and store 8% NaHCO3 solution under a N2-CO2 (80:20, v/v) gas mixture.